Publications

Moore group - University of Kent (2018-2022) and Queen Mary University of London (2023).


25. Kameshwari Chengan, Charlotte Hind, Maria Stanley, Matthew E. Wand, Lakshmeesha Nagappa, Kevin Howland, Tanith Hanson, Rubén Martín-Escolano, Anastasios D. Tsaousis, José A. Bengoechea, J. Mark Sutton, Christopher M. Smales and Simon J. Moore. A cell-free strategy for host-specific profiling of intracellular antibiotic sensitivity and resistance. npj Antimicrobials and Resistance (in press)


24. Simon J Moore, Hung-En Lai, Jian Li and  Paul S Freemont. Streptomyces cell-free systems for natural product discovery and engineering (2023). Natural Products Reports 40, 228-236


23. Lakshmeesha K Nagappa, Wakana Sato, Farzana Alam, Kameshwari Chengan, Christopher M Smales, T Der Haar Von, Karen M Polizzi, Katarzyna P Adamala, Simon J Moore. A ubiquitous amino acid source for prokaryotic and eukaryotic cell-free transcription-translation systems (2022). Frontiers in Biotechnology and Bioengineering

23. Agata Kennedy, Guy Griffin, Paul S Freemont, Karen M Polizzi, Simon J Moore. A curcumin direct protein (DiPro) biosensor for cell-free prototyping (2022) Engineering Biology, 6(2):62-68

22. Rebekka Biedendieck, Tobias Knuuti, Simon J Moore and Dieter Jahn. The “beauty in the beast”—the multiple uses of Priestia megaterium in biotechnology (2021) Applied Microbiology and Biotechnology 105:5719–5737


21. Jian Li, Yong-Chan Kwon, Yuan Lu, Simon J Moore*. Editorial: Cell-Free Synthetic Biology (2021) Frontiers in Bioengineering and Biotechnology 9:1210.


20. Agata Kennedy, Guy Griffin, Paul S Freemont, Karen M Polizzi, Simon J Moore. A curcumin direct protein (DiPro) biosensor for cell-free prototyping (2022) bioRxiv 2021.09.22.461347


19. Hung-En Lai, Alan M. C. Obled, Soo Mei Chee, Rhodri M. Morgan, Rosemary Lynch, Sunil V. Sharma, Simon J. Moore, Karen M. Polizzi, Rebecca J. M. Goss*, Paul S. Freemont. A GenoChemetic strategy for derivatization of the violacein natural product scaffold (2021) ACS Chemical Biology 16(11):2116.


18. Ming Toh, Kameshwari Chengan, Tanith Hanson, Paul S Freemont, Simon J Moore. A high-yield Streptomyces transcription-translation toolkit for synthetic biology and natural product applications (2021) JoVE 10.3791/63012


17. Simon J Moore. Enzyme alchemy: cell-free synthetic biochemistry for natural products (2019) Emerging Topics in Biosciences 3: 529-535


PhD to Postdoctoral papers - 2013-2018


16. Simon J Moore*, Hung-En Lai, Soo-Mei Chee, Ming Toh, Seth Coode, Kameshwari Chengan, Patrick Capel, Christophe Corre, Emmanuel Lc de Los Santos, Paul S Freemont*. A Streptomyces cell-free Toolkit for Synthetic Biology (2021) ACS Synth Biol 10: 402-411


15. Simon J Moore, Tommaso Tosi, David Bell, Yonek B Hleba, Karen M Polizzi, Paul S Freemont. High-yield ‘one-pot’ biosynthesis of raspberry ketone, a high-value fine chemical (2021) Synth. Biol. 6:ysab021


14. Simon J Moore, Yonek B Hleba, Sarah Bischoff, David Bell, Karen M Polizzi, Paul S Freemont. Refactoring of a Synthetic Raspberry Ketone Pathway with EcoFlex (2021) Microb. Cell Factories 20: 1-11


13. Hung-En Lai, Caoimhe Canavan, Loren Cameron, Simon Moore, Monika Danchenko, Todd Kuiken, Zuzana Sekeyová, Paul S Freemont. Synthetic biology and the United Nations (2019) Trends in Biotechnology 37: 1146-1151


12. Simon J Moore, James T MacDonald, Sarah Wienecke, Alka Ishwarbhai, Argyro Tsipa, Rochelle Aw, Nicolas Kylilis, David J Bell, David W McClymont, Kirsten Jensen, Karen M Polizzi, Rebekka Biedendieck, Paul S Freemont. Rapid acquisition and model-based analysis of cell-free transcription-translation reactions from non-model bacteria (2018) PNAS 115: E4340-E4349


11. Simon J Moore, James T MacDonald and Paul S Freemont. Cell-free synthetic biology for in vitro prototype engineering (2017) Biochemical Society Transactions 45: 785-791.


10. Simon J Moore, Sven T Sowa, Christopher Schuchardt, Evelyne Deery, Andrew D Lawrence, José Vazquez Ramos, Susan Billig, Claudia Birkemeyer, Peter T Chivers, Mark J Howard, Stephen EJ Rigby, Gunhild Layer, Martin J Warren. Elucidation of the biosynthesis of the methane catalyst coenzyme F430 (2017) Nature 543: 78-82


9. Simon J Moore, Hung‐En Lai, Hannah Needham, Karen M Polizzi, Paul S Freemont. Streptomyces venezuelae TX-TL – a next generation cell-free synthetic biology tool (2017) Biotechnol J DOI: 10.1002/biot.201600678


8. Simon J Moore, Hung-En Lai, Richard JR Kelwick, Soo Mei Chee, David J Bell, Karen M Polizzi, Paul S Freemont. EcoFlex: A multifunctional toolkit for E. coli synthetic biology (2016) ACS Synth Biol 5: 1059-1069


7. Margarita B Kopniczky, Simon J Moore, Paul S Freemont. Multilevel regulation and translational switches in synthetic biology (2015) IEEE Transactions 9: 485-496


6. Simon J Moore, Matthias J Mayer, Rebekka Biedendieck, Evelyne Deery, Martin J Warren. Towards a cell factory for vitamin B12 production in Bacillus megaterium: Bypassing of the cobalamin riboswitch control elements (2014) New Biotechnology 31: 553-61


5. Simon J Moore, Andrew D Lawrence, Rebekka Biedendieck, Evelyne Deery, Stefanie Frank, Mark J Howard, Stephen EJ Rigby, Martin J Warren. Elucidation of the anaerobic pathway for the corrin component of cobalamin (vitamin B12) (2013) PNAS 110: 14906-14911


4. Simon J Moore, Rebekka Biedendieck, Andrew D Lawrence, Evelyne Deery, Mark J Howard, Stephen EJ Rigby, Martin J Warren. Characterization of the enzyme CbiH60 involved in anaerobic ring contraction of the cobalamin (vitamin B12) biosynthetic pathway (2013) Journal of Biological Chemistry 288: 297-305


3. Simon J Moore and Martin J Warren. The anaerobic biosynthesis of vitamin B12 (2012) Biochemical Society Transactions 40: 581-586

 

Book Chapters


2. Hung-En Lai, Simon Moore, Karen Polizzi, Paul Freemont. EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology (2018) Springer Protocols: Synthetic Biology p429-444


1. Evelyne Deery, Stefanie Frank, Andrew Lawrence, Simon Moore, Susanne Schroeder, Martin J Warren. Synthetic biology and a new era in metabolic engineering: From complex biochemical pathways to compartmentalized metabolic processes – a vitamin connection (2014) Encyclopedia of Molecular Cell Biology and Molecular Medicine, Wiley